Kodi mumagwira ntchito yofufuza zamankhwala?Ngati ndi choncho, ndiye kuti munamvapo za Sulfo-NHS.Pamene ntchito yofunika kwambiri ya chigawochi mu kafukufuku ikupitiriza kudziwika, gululi likulowa m'ma laboratories ambiri padziko lonse lapansi.M'nkhaniyi, tikambirana za Sulfo-NHS ndi chifukwa chake ndi chida chofunikira kwambiri kwa iwo omwe amaphunzira sayansi yachilengedwe.
Choyamba, kodi Sulfo-NHS ndi chiyani?Dzinali ndi lalitali pang'ono, choncho tiyeni tidutse.Sulfo amaimira sulfonic acid ndipo NHS imayimira N-hydroxysuccinimide.Zinthu ziwirizi zikaphatikizana,Sulfo-NHSamapangidwa.Pagululi lili ndi ntchito zingapo pakufufuza zamankhwala, koma chimodzi mwazinthu zake zazikulu ndikutha kuyika mapuloteni mwa kusankha.
Sulfo-NHS imagwira ntchito pochita ndi ma amines oyambira (ie -NH2 magulu) pamaketani am'mbali a zotsalira za lysine m'mapuloteni.Kwenikweni, Sulfo-NHS imaphatikiza mapuloteni a "tag", kuwapangitsa kukhala kosavuta kuzindikira ndi kusanthula muzoyesa zosiyanasiyana.Izi zapangitsa kuti madera ambiri afukufuku athe kupita patsogolo molondola komanso mwatsatanetsatane.
Ndiye, kodi Sulfo-NHS imagwiritsidwa ntchito bwanji?Kugwiritsidwa ntchito kofala kwa mankhwalawa ndi kafukufuku wa immunology.Sulfo-NHS yawonetsedwa kuti imalemba bwino ma antibodies ndi ma antigen, ndikutsegula njira zatsopano zophunzirira zovuta za chitetezo chamthupi ndi matenda.Kuonjezera apo,Sulfo-NHSangagwiritsidwe ntchito m'maphunziro okhudzana ndi mapuloteni-mapuloteni chifukwa amalola ochita kafukufuku kuti azindikire mofulumira komanso mosavuta pamene mapuloteni awiri amagwirizana.
Malo ena omwe Sulfo-NHS amagwiritsidwa ntchito kwambiri ndi a proteinomics.Proteomics amaphunzira kapangidwe ndi ntchito ya mapuloteni onse m'thupi, ndiSulfo-NHSndi chida chofunikira pakuwunikaku.Polemba ma protein omwe ali ndi Sulfo-NHS, ofufuza amatha kuyesa kuti adziwe zambiri za proteome ya chamoyo chomwe chapatsidwa, chomwe chingathandize kuzindikira zomwe zingayambitse matenda.
Sulfo-NHS imathandizanso pakupanga mankhwala atsopano.Ofufuza akamayesa kupanga mankhwala atsopano, ndikofunikira kuwonetsetsa kuti akuloza puloteni yomwe akufuna osati mapuloteni ena aliwonse m'thupi.Pogwiritsa ntchitoSulfo-NHSkuti musankhe mapuloteni, ofufuza amatha kuzindikira zolinga zenizeni za mankhwala omwe angathe, zomwe zingathandize kufulumizitsa ndondomeko ya chitukuko cha mankhwala.
Ndiye muli nazo izo!Sulfo-NHS sangakhale mawu odziwika bwino kunja kwa gulu la asayansi, koma gululi likukhala chida chofunikira kwambiri pakufufuza zamankhwala.Kuchokera kufukufuku wa immunology kupita ku proteomics kupita ku chitukuko cha mankhwala, Sulfo-NHS ikuthandiza ofufuza kuti apite patsogolo kwambiri m'maderawa ndipo ndife okondwa kuona zomwe atulukira pambuyo pake.
Nthawi yotumiza: Jun-12-2023